A solution to combat Erythromycin-resistant bacteria isolated from returned activated sludge in Salt Lake City, Utah

Noor Un-Nisa; Ayesha Tajammul

doi:10.58921/jse.01.01.017

Noor Un-Nisa Water, Sanitation and Health Sciences (WASH), U.S Pakistan Center for Advanced Studies in Water (USPCAS-W), Mehran University of Engineering and Technology, Jamshoro, Pakistan
Ayesha Tajammul

DOI: https://doi.org/10.58921/jse.01.01.017

Abstract

Treatments offered in clinics are now ineffective due to antibiotic resistance in particular bacterial strains. Plant-based antibiotics are in high demand in developing and developed countries; they are common medications that are simple to use, pose no environmental risks, have no adverse side effects, and are competitively priced. This study aimed to screen plant-based medicine against Erythromycin-resistant bacteria such as E. coli, Enterobacter, Enterococcus, and E. Faecalis. The disk diffusion method and the agar well diffusion method were used to determine the zone of inhibition of Coriandrum Sativum L. (coriander), Zingiber officinale (ginger), and Spinacia oleracea (spinach). Minimum inhibitory concentration was evaluated via UV-visible Spectrophotometry at 600nm, while Polymerase Chain Reaction and Electrophoresis identified genomic activity for ErmB. Coriander was found to be the most effective against Erythromycin-resistant bacteria, and ErmB genes were found in almost all of the isolates.

Keywords: Coriandrum Sativum, Zingiber officinale, Spinacia oleracea, Antimicrobial activity, and Erythromycin